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Becton Dickinson lsrfortessa x-20 multi-dimensional high-definition flow cytometry
Lsrfortessa X 20 Multi Dimensional High Definition Flow Cytometry, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GraphPad Software Inc high-dimensional flow cytometry
(A,B) Variation in colonic tumorigenic potential in GF ApcMin/+ mice at 12-14 wk post-inoculation (p.i.) by (A) individual biofilm-positive tumor (BF+T) or (B) biofilm-negative tumor (BF−T) CRC mucosal samples from 6 patients. N = 6-8 mice per group, from 2 independent experiments. (C) 16S rRNA amplicon sequencing relative abundances of the top 20 species in the 6 patient tumor slurry inocula and 12-14 wk p.i. stools from mice gavaged with the tumor slurries. (D) A consortium of 30 bacterial isolates derived from GF mice gavaged with the 3728T slurry recapitulated colonic <t>tumorigenesis</t> of the original 3728T slurry. N = 5-11 mice per group. (E-F) Colonic tumor counts (E) and representative methylene blue staining (F) of colonic tumors (arrows) at 12 wk p.i. in a vancomycin/gentamicin model (see Methods) of chronic colonization with non-toxigenic (TcdA−TcdB−) or toxigenic (TcdA+TcdB+) C. difficile strains. N = 5-18 mice per group, from two independent experiments. (G-H) Colonic tumor counts (G) and representative methylene blue staining (H) of colonic tumors (arrows) from GF ApcMin/+ mice gavaged with the 3728T isolates with or without the CIm_3728T or CIm_3752T C. difficile strains. N = 6-8 mice per group. (I) Colonic tumors induced in GF ApcMin/+ mice gavaged with either the 3979T slurry or the 3979T slurry with C. difficile strain CIm_3728T at 10 wk p.i. Cross represents a mouse death. N = 7-8 mice per group. Statistical significance was calculated via Kruskal-Wallis followed by Mann-Whitney tests. Mann-Whitney p-values are shown.
High Dimensional Flow Cytometry, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high-dimensional+flow+cytometry/pmc09357196-464-0-10?v=GraphPad+Software+Inc
Average 90 stars, based on 1 article reviews
high-dimensional flow cytometry - by Bioz Stars, 2026-06
90/100 stars
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(A,B) Variation in colonic tumorigenic potential in GF ApcMin/+ mice at 12-14 wk post-inoculation (p.i.) by (A) individual biofilm-positive tumor (BF+T) or (B) biofilm-negative tumor (BF−T) CRC mucosal samples from 6 patients. N = 6-8 mice per group, from 2 independent experiments. (C) 16S rRNA amplicon sequencing relative abundances of the top 20 species in the 6 patient tumor slurry inocula and 12-14 wk p.i. stools from mice gavaged with the tumor slurries. (D) A consortium of 30 bacterial isolates derived from GF mice gavaged with the 3728T slurry recapitulated colonic tumorigenesis of the original 3728T slurry. N = 5-11 mice per group. (E-F) Colonic tumor counts (E) and representative methylene blue staining (F) of colonic tumors (arrows) at 12 wk p.i. in a vancomycin/gentamicin model (see Methods) of chronic colonization with non-toxigenic (TcdA−TcdB−) or toxigenic (TcdA+TcdB+) C. difficile strains. N = 5-18 mice per group, from two independent experiments. (G-H) Colonic tumor counts (G) and representative methylene blue staining (H) of colonic tumors (arrows) from GF ApcMin/+ mice gavaged with the 3728T isolates with or without the CIm_3728T or CIm_3752T C. difficile strains. N = 6-8 mice per group. (I) Colonic tumors induced in GF ApcMin/+ mice gavaged with either the 3979T slurry or the 3979T slurry with C. difficile strain CIm_3728T at 10 wk p.i. Cross represents a mouse death. N = 7-8 mice per group. Statistical significance was calculated via Kruskal-Wallis followed by Mann-Whitney tests. Mann-Whitney p-values are shown.

Journal: Cancer discovery

Article Title: Human colon cancer-derived Clostridioides difficile strains drive colonic tumorigenesis in mice

doi: 10.1158/2159-8290.CD-21-1273

Figure Lengend Snippet: (A,B) Variation in colonic tumorigenic potential in GF ApcMin/+ mice at 12-14 wk post-inoculation (p.i.) by (A) individual biofilm-positive tumor (BF+T) or (B) biofilm-negative tumor (BF−T) CRC mucosal samples from 6 patients. N = 6-8 mice per group, from 2 independent experiments. (C) 16S rRNA amplicon sequencing relative abundances of the top 20 species in the 6 patient tumor slurry inocula and 12-14 wk p.i. stools from mice gavaged with the tumor slurries. (D) A consortium of 30 bacterial isolates derived from GF mice gavaged with the 3728T slurry recapitulated colonic tumorigenesis of the original 3728T slurry. N = 5-11 mice per group. (E-F) Colonic tumor counts (E) and representative methylene blue staining (F) of colonic tumors (arrows) at 12 wk p.i. in a vancomycin/gentamicin model (see Methods) of chronic colonization with non-toxigenic (TcdA−TcdB−) or toxigenic (TcdA+TcdB+) C. difficile strains. N = 5-18 mice per group, from two independent experiments. (G-H) Colonic tumor counts (G) and representative methylene blue staining (H) of colonic tumors (arrows) from GF ApcMin/+ mice gavaged with the 3728T isolates with or without the CIm_3728T or CIm_3752T C. difficile strains. N = 6-8 mice per group. (I) Colonic tumors induced in GF ApcMin/+ mice gavaged with either the 3979T slurry or the 3979T slurry with C. difficile strain CIm_3728T at 10 wk p.i. Cross represents a mouse death. N = 7-8 mice per group. Statistical significance was calculated via Kruskal-Wallis followed by Mann-Whitney tests. Mann-Whitney p-values are shown.

Article Snippet: Tumorigenesis, high-dimensional flow cytometry, and IHC statistics were performed in GraphPad Prism version 9.1.

Techniques: Amplification, Sequencing, Derivative Assay, Staining, MANN-WHITNEY